ADVANCED MICROMANIPULATION

Intracytoplasmic Sperm Injection (ICSI)

High-magnification micromanipulation technique designed to overcome severe male factor infertility and previous fertilization challenges.

90%+

Fertilization Rate

400x

Laser Magnification

Direct

Single Sperm Injection

Proven

Male Factor Solution

Understanding the Procedure & Clinical Approach

Intracytoplasmic Sperm Injection (ICSI) is a sophisticated micromanipulation procedure used alongside IVF. Rather than placing thousands of sperm around an egg and waiting for natural penetration, an experienced senior embryologist selects a single, morphologically optimal sperm under high magnification and injects it directly into the cytoplasm of a mature egg using an ultra-fine glass micropipette.

ICSI has revolutionized the treatment of severe male infertility, making biological parenthood possible even in cases of extremely low sperm counts or surgically extracted sperm.

Step-by-Step Treatment Protocol

01

Oocyte Denudation

Retrieved eggs are prepared by removing surrounding cumulus cells to accurately assess maturity (Metaphase II).

02

Sperm Selection Under Magnification

High-power inverted microscope optics identify sperm with optimal motility and head/tail morphology.

03

Micro-Injection

The selected sperm is immobilized and microinjected directly into the mature oocyte with sub-micron precision.

04

Embryo Culture & Assessment

Fertilization is confirmed 16-18 hours post-injection, followed by multi-day incubation to the blastocyst stage.

Who is an Ideal Candidate?

  • ✓ Severe oligozoospermia (extremely low sperm count)
  • ✓ Asthenozoospermia (poor sperm motility) or teratozoospermia (abnormal morphology)
  • ✓ Obstructive or non-obstructive azoospermia (using surgically retrieved TESA/PESA sperm)
  • ✓ Previous poor or failed fertilization with standard IVF
  • ✓ Cryopreserved or frozen oocyte cycles

Frequently Asked Questions

Is ICSI safe for the developing baby?

Decades of global clinical data show that children conceived via ICSI have health and development profiles completely comparable to naturally conceived children.

Can ICSI be done with surgically extracted sperm?

Yes, ICSI is the gold standard when using sperm retrieved via PESA (Percutaneous Epididymal Sperm Aspiration) or TESA (Testicular Sperm Aspiration).

Dr. Prathima Kamisetty

Clinical Director & Fertility Specialist

Quick Treatment Facts

Timeline: Integrated with IVF Cycle

Hospital Stay: Outpatient

Location: Ramesh Hospitals, Ongole

Ready to discuss ICSI Treatment?

Take the first step with confidence. Book your consultation with Dr. Prathima Kamisetty at Komali Fertility Centre Ongole.

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